Coagulation factors II,V and methylene tetrahydrofolate reductase gene polymorphism in patients with diabetic nephropathy: prevalence, clinical and prognostic implications
https://doi.org/10.14341/2072-0351-6009
Abstract
Aim.
To study prevalence, clinical and prognostic significance of prothrombotic genotypes pre-dominant in inborn thrombophilia in patients with diabeticnephropathy (DN).
Materials and methods.
A total of 90 patients with DN were examined; 54 and 36 cases suffered DM1 and DM2 respectively. Control group comprised100 healthy subjects. PCR was used to iden-tify single nucleotide substitution (C677T) in the methylene tetrahydrofolate reductase gene (MTHFR),point mutation in coagulation factor V gene (FV), and G202210A mutation in factor II gene (FII).
Results.
The probability of DN in patients with DM1 increases in the presence of Leiden mutation and in DM2 patients in the presence of single nucleotidesubstitu-tion (C677T) in MTHFR gene and G202210A mutation in the 3-untranslated region of FII.
Conclusion.
The prevalence of the above mutations associated with blood coagulation potential in DN patients is higher than in healthy subjects.Key words: diabetes mellitus, diabetic nephropathy, gene polymorphism, methylenetetrahydrofolate reductase
To study prevalence, clinical and prognostic significance of prothrombotic genotypes pre-dominant in inborn thrombophilia in patients with diabeticnephropathy (DN).
Materials and methods.
A total of 90 patients with DN were examined; 54 and 36 cases suffered DM1 and DM2 respectively. Control group comprised100 healthy subjects. PCR was used to iden-tify single nucleotide substitution (C677T) in the methylene tetrahydrofolate reductase gene (MTHFR),point mutation in coagulation factor V gene (FV), and G202210A mutation in factor II gene (FII).
Results.
The probability of DN in patients with DM1 increases in the presence of Leiden mutation and in DM2 patients in the presence of single nucleotidesubstitu-tion (C677T) in MTHFR gene and G202210A mutation in the 3-untranslated region of FII.
Conclusion.
The prevalence of the above mutations associated with blood coagulation potential in DN patients is higher than in healthy subjects.Key words: diabetes mellitus, diabetic nephropathy, gene polymorphism, methylenetetrahydrofolate reductase
About the Authors
Olga Filippovna Sibireva
Siberian State Medical University, Tomsk
Ekaterina Yur'evna Khitrinskaya
Siberian State Medical University, Tomsk
Vadim Vital'evich Kalyuzhin
Siberian State Medical University, Tomsk
Aleksey Eduardovich Sazonov
Siberian State Medical University, Tomsk
Igor Ivanovich Ivanchuk
Siberian State Medical University, Tomsk
Viktoriya Yur'evna Grankina
Siberian State Medical University, Tomsk
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Review
For citations:
Sibireva O.F., Khitrinskaya E.Yu., Kalyuzhin V.V., Sazonov A.E., Ivanchuk I.I., Grankina V.Yu. Coagulation factors II,V and methylene tetrahydrofolate reductase gene polymorphism in patients with diabetic nephropathy: prevalence, clinical and prognostic implications. Diabetes mellitus. 2010;13(1):6-9. (In Russ.) https://doi.org/10.14341/2072-0351-6009
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