Markers of apoptosis in patients with type 1 diabetes mellitus at the onset of the disease
https://doi.org/10.14341/2072-0351-5711
Abstract
Aim.
This work was to study activation markers of apoptosis (CD95, CD95L) on peripheral blood lymphocytes of patients with type 1 diabetes mellitus (DM1) at the onset of the disease.
Materials and methods.
A total of 33 patients (25 men and 8 women) divided into 2 groups were available for biochemical, genetic, and immunological examination.
Results.
Those with the ?classical? onset of DM1 had significantly fewer CD95-expressing lymphocytes and more CD95L+ lymphocytes than control subjects. Carriers of genes responsible for high risk of DM1 showed especially large number of lymphocytes expressing CD95L.
Conclusion.
It is conjectured that DM1 is characterized by the suppression of mechanism controlling apoptosis of activated lymphocytes that may promote prolongation of the immune response.
This work was to study activation markers of apoptosis (CD95, CD95L) on peripheral blood lymphocytes of patients with type 1 diabetes mellitus (DM1) at the onset of the disease.
Materials and methods.
A total of 33 patients (25 men and 8 women) divided into 2 groups were available for biochemical, genetic, and immunological examination.
Results.
Those with the ?classical? onset of DM1 had significantly fewer CD95-expressing lymphocytes and more CD95L+ lymphocytes than control subjects. Carriers of genes responsible for high risk of DM1 showed especially large number of lymphocytes expressing CD95L.
Conclusion.
It is conjectured that DM1 is characterized by the suppression of mechanism controlling apoptosis of activated lymphocytes that may promote prolongation of the immune response.
About the Authors
Elena Vladimirovna Pekareva
Endocrinology Research Centre, Moscow
Tatiana Vasil'evna Nikonova
Endocrinology Research Centre, Moscow
Vera Anatol'evna Gorelysheva
Endocrinology Research Centre, Moscow
Yana Stanislavovna Zvereva
Endocrinology Research Centre, Moscow
Svetlana Mikhailovna Stepanova
Endocrinology Research Centre, Moscow
Sergey Alexandrovich Prokof'ev
Endocrinology Research Centre, Moscow
Olga Mikhailovna Smirnova
Endocrinology Research Centre, Moscow
References
1. Moulian N., Berrih-Aknin S. Fas/APO-1/CD95 in health and autoimmune disease: thymic and peripheral aspects // Semin Immunol. - 1998. - 10 (6). - Р.449-456.
2. Gronski M.A., Weinem M. Death pathways in T cell homeostasis and their role in autoimmune diabetes // Rev. Diabetic. Stud. - 2006. - 3 (2). - Р.88-95.
3. Kabelitz D., Pohl T., Pechhold K. Activation-induced cell death (apoptosis) of mature peripheral T lymphocytes // Immunol. Today, 1993, 14 - Р. 338-339.
4. Дедов И.И., Никонова Т.В., Смирнова О.М. и др. Роль цитокинов в регуляции иммунного ответа и механизмы гибели -клеток при раз- личных вариантах течения сахарного диабета типа 1 // Проблемы эндокринологии. - 2005. - том 51, №3. - С. 3-7.
5. Giordano C., De Maria R., Stassi G. et al. Defective expression of the apoptosiss-inducing CD95 (Fas/APO-1) molecule on T- and B-cells in IDDM // Diabetologia. - 1995. - 38. - Р.1449-1454.
6. DeFranco S., Bonissoni S., Cerutti F. et al. Defective function of Fas in patients with type 1 diabetes associated with other autoimmune diseases // Diabetes. - 2001. - 50 - Р.483-488.
7. World Health Organization: Definition, Diagnosis, and Classification of Diabetes Mellitus and Its Complications: Report of a WHO consultation. Part 1: Diagnosis and Classification of Diabetes Mellitus. Geneva, World Health Organization, 1999 (WHO/NCD/NCS/99.2).
8. Ройт А., Бростофф Дж., Мейл Д. Иммунология. - Москва, 2000. - 582 с.
9. Барышников А.Ю., Шишкин Ю.В. Иммунологические проблемы апоптоза. - Москва. - 2002. - 320 с.
10. Moriwaki M., Itoh N., Miyagawa J. et al. Fas and Fas ligand expression in inflamed islets in pancreas sections of patients with recent-onset type I diabetes mellitus // Diabetologia. - 1999. - 42 - Р.1332-1340.
11. Darville M.I., Liu D., Chen M-C. et al. Molecular regulation of Fas expression in -cells // Diabetes. - 2001. - 50 (Suppl. 1) - Р.83.
12. Потапнев М.П. Апоптоз клеток иммунной системы и его регуляция цитокинами // Иммунология. - 2002. - №4. - С.237-242.
Review
For citations:
Pekareva E.V., Nikonova T.V., Gorelysheva V.A., Zvereva Ya.S., Stepanova S.M., Prokof'ev S.A., Smirnova O.M. Markers of apoptosis in patients with type 1 diabetes mellitus at the onset of the disease. Diabetes mellitus. 2009;12(4):73-89. (In Russ.) https://doi.org/10.14341/2072-0351-5711
Views:
625
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0).
Email this article 
