Class II HLA diabetogenic markers in an Udmurtian population: genotype dependence, the role of DQ trans-heterodimers
https://doi.org/10.14341/2072-0351-5449
Abstract
Aim.
To analyse association of class II HLA genotype (DRB1, DQA1, DQB1) with type 1 diabetes mellitus (DM1) in an Udmurtian population.
Materials and methods.
The case-control method was applied in the study involving 29 children with DM1 and 97 age-matched healthy subjects. HLAalleles were identified by multiprimer allele-specific PCR. Association with DM1 was evaluated from the OR-odds ratio. Calculations were made usingStatSoft and STATISTICA 6 programs.
Results.
The occurrence of ?classical? highly predisposing haplotypes in the studied Udmurtian population proved significantly lower than in otherCaucasoid populations (DRB1*04-DQA1*0301-DQB1*0302 - 2,6 vs 8-16% and DRB1*17(03)-DQA*0501-DQB1*0201 - 3,6 vs 6-12,9%)trans-Encoded DQ heterodimers (DQA1*0301-DQB1*0201 and DQA1*0301-DQB1*0302) were shown to play the key role in determining the riskof DM1. They were found in 62,1% of the patients compared with 10,3% of control subjects (OR=14,2; pc=6Ч10-5).
Conclusion.
Positive DQA1*0301-DQB1*0302 or/and *0201 genotype is the most sensitive predictor of DM1 in the studied Udmurtian population.
To analyse association of class II HLA genotype (DRB1, DQA1, DQB1) with type 1 diabetes mellitus (DM1) in an Udmurtian population.
Materials and methods.
The case-control method was applied in the study involving 29 children with DM1 and 97 age-matched healthy subjects. HLAalleles were identified by multiprimer allele-specific PCR. Association with DM1 was evaluated from the OR-odds ratio. Calculations were made usingStatSoft and STATISTICA 6 programs.
Results.
The occurrence of ?classical? highly predisposing haplotypes in the studied Udmurtian population proved significantly lower than in otherCaucasoid populations (DRB1*04-DQA1*0301-DQB1*0302 - 2,6 vs 8-16% and DRB1*17(03)-DQA*0501-DQB1*0201 - 3,6 vs 6-12,9%)trans-Encoded DQ heterodimers (DQA1*0301-DQB1*0201 and DQA1*0301-DQB1*0302) were shown to play the key role in determining the riskof DM1. They were found in 62,1% of the patients compared with 10,3% of control subjects (OR=14,2; pc=6Ч10-5).
Conclusion.
Positive DQA1*0301-DQB1*0302 or/and *0201 genotype is the most sensitive predictor of DM1 in the studied Udmurtian population.
About the Authors
Olga Nikolavna Ivanova
Endocrinological Research Centre, Moscow
Sergey Alexandrovich Prokof'ev
Endocrinological Research Centre, Moscow
Yana Stanislavovna Zvereva
Endocrinological Research Centre, Moscow
Tatiana Viktorovna Kovalenko
Izhevsk State Medical Academy, Izhevsk
Andrey Vladimirovich Blinov
Izhevsk State Medical Academy, Izhevsk
Valentina Alexandrovna Peterkova
Endocrinological Research Centre, Moscow
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Review
For citations:
Ivanova O.N., Prokof'ev S.A., Zvereva Ya.S., Kovalenko T.V., Blinov A.V., Peterkova V.A. Class II HLA diabetogenic markers in an Udmurtian population: genotype dependence, the role of DQ trans-heterodimers. Diabetes mellitus. 2009;12(3):33-36. (In Russ.) https://doi.org/10.14341/2072-0351-5449
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