Associations of vascular endothelial growth factor (VEGF) gene and cytokine (IL-1B, IL-4, IL-6, IL-10, TNFA) genes combinations with type 2 diabetes mellitus in women
Abstract
Aim.
To study the association between vascular endothelial growth factor (VEGF) and cytokine (IL1B, IL4, IL6, IL10 and TNFA)gene polymorphism combinations with type 2 diabetes mellitus (T2DM) in women.
Materials and methods.
374 Caucasian women without carbohydrate metabolism disorders from 23 to 68 years of age and 212 womenwith T2DM from 28 to 69 years of age were included in the study. The combinations of polymorphism А-2578С, С+936Т in VEGFgene with polymorphism in IL1B С-31Т, IL4 С-590Т, IL6 G-174C, IL10 A-592C and А-1082G, TNFA А-238G, A-308G and A-863Cwere studied.
Results.
Analysis revealed 52 combined genetic variations with different rate of occurrence between diabetic and control groups(р<0.002). Among variations positively associated with T2DM (n=34) high frequency of homozygote genotypes of VEGF -2578CC and+936CC, IL4 -590СС, IL6 -174GG, IL10 -592СC and -1082АА, TNFA -238GG, -308GG and -863СC, was observed. In 18 combinedgenetic variations that were negatively associated with T2DM, homozygous variant of VEGF and IL10 were equally distributed withheterozygous genotypes, while heterozygote IL1B, IL4 and IL6 genotypes were more prevalent.
Conclusion.
Combination of VEGF gene polymorphisms A-2578С and C+936Т with polymorphism in IL1B C-31Т, IL4 С-590Т, IL6G-174C, IL10 А-592C and А-1082G, TNFA A-238G, A-308G and A-863C may be genetic risk factors for T2DM in Caucasian women.
To study the association between vascular endothelial growth factor (VEGF) and cytokine (IL1B, IL4, IL6, IL10 and TNFA)gene polymorphism combinations with type 2 diabetes mellitus (T2DM) in women.
Materials and methods.
374 Caucasian women without carbohydrate metabolism disorders from 23 to 68 years of age and 212 womenwith T2DM from 28 to 69 years of age were included in the study. The combinations of polymorphism А-2578С, С+936Т in VEGFgene with polymorphism in IL1B С-31Т, IL4 С-590Т, IL6 G-174C, IL10 A-592C and А-1082G, TNFA А-238G, A-308G and A-863Cwere studied.
Results.
Analysis revealed 52 combined genetic variations with different rate of occurrence between diabetic and control groups(р<0.002). Among variations positively associated with T2DM (n=34) high frequency of homozygote genotypes of VEGF -2578CC and+936CC, IL4 -590СС, IL6 -174GG, IL10 -592СC and -1082АА, TNFA -238GG, -308GG and -863СC, was observed. In 18 combinedgenetic variations that were negatively associated with T2DM, homozygous variant of VEGF and IL10 were equally distributed withheterozygous genotypes, while heterozygote IL1B, IL4 and IL6 genotypes were more prevalent.
Conclusion.
Combination of VEGF gene polymorphisms A-2578С and C+936Т with polymorphism in IL1B C-31Т, IL4 С-590Т, IL6G-174C, IL10 А-592C and А-1082G, TNFA A-238G, A-308G and A-863C may be genetic risk factors for T2DM in Caucasian women.
About the Authors
Vladimir Iosifovich Konenkov
Research Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
Alla Vladimirovna Shevchenko
Research Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
Victor Fedorovich Prokof'ev
Research Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
Vadim Valer'evich Klimontov
Research Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
Maksim Alexandrovich Korolev
Research Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
Olga Nikolaevna Fazullina
Research Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
Svetlana Alexandrovna Lapsina
Research Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
Elena Anatol'evna Koroleva
Research Institute of Clinical and Experimental Lymphology, Novosibirsk, Russian Federation
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Review
For citations:
Konenkov V.I., Shevchenko A.V., Prokof'ev V.F., Klimontov V.V., Korolev M.A., Fazullina O.N., Lapsina S.A., Koroleva E.A. Associations of vascular endothelial growth factor (VEGF) gene and cytokine (IL-1B, IL-4, IL-6, IL-10, TNFA) genes combinations with type 2 diabetes mellitus in women. Diabetes mellitus. 2012;15(3):4-10. (In Russ.)
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