Genetic markers of insulin resistance in gestational diabetes
https://doi.org/10.14341/2072-0351-5702
Abstract
Aim.
To search for genetic markers of insulin resistance and impaired insulin secretion in pregnant women with gestational diabetes mellitus (GDM).
Materials and methods.
A total of 100 healthy pregnant women and 185 patients with GDM were available for examination. 80 patients developedGDM during current pregnancy, in 105 it was diagnosed 4-19 years ago. 25 of the 105 GDM patients had a history of type 2 DM. The following parameterswere measured: beta-cell secretory activity (proinsulin, ITI, C-peptide), total cholesterol (CH), HDL and LDL CH, triglycerides, HbA1c,fasting glycemia. Molecular-genetic DNA testing using PCR included studies of KCNJ 11, TCF7L2, PPARG2, ADIPOQ, ADIPOR1, ADIPOR2gene polymorphism. These genes were chosen based on the published data associating them with disturbed insulin secretion and sensitivity in DM2patient.
Results.
Pregnant women with GDM and obesity showed elevated IRI and leptin levels compared with controls. This rise was accompanied bymarked insulin resistance in 75% of these patients. In 50% of the healthy women proinsulin and insulin secretion decreased. Obesity in pregnantpatients was associated with significant elevation of proinsulin, IRI, and C-peptyide levels and GDM with Lys/Lys genotype of polymorphous markerGlu23k of KCNJ11 gene, pro and ala allele of polymorphous marker A219T of ADIPOR2 gene. These associations suggest specific genetic featuresof GDM related to impaired insulin secretion and sensitivity.
Conclusion.
Studies of common genetic nature of GDM and DM2 permit to identify risk groups at the preclinical stage, plan prevention and treatmentof these disorders.
To search for genetic markers of insulin resistance and impaired insulin secretion in pregnant women with gestational diabetes mellitus (GDM).
Materials and methods.
A total of 100 healthy pregnant women and 185 patients with GDM were available for examination. 80 patients developedGDM during current pregnancy, in 105 it was diagnosed 4-19 years ago. 25 of the 105 GDM patients had a history of type 2 DM. The following parameterswere measured: beta-cell secretory activity (proinsulin, ITI, C-peptide), total cholesterol (CH), HDL and LDL CH, triglycerides, HbA1c,fasting glycemia. Molecular-genetic DNA testing using PCR included studies of KCNJ 11, TCF7L2, PPARG2, ADIPOQ, ADIPOR1, ADIPOR2gene polymorphism. These genes were chosen based on the published data associating them with disturbed insulin secretion and sensitivity in DM2patient.
Results.
Pregnant women with GDM and obesity showed elevated IRI and leptin levels compared with controls. This rise was accompanied bymarked insulin resistance in 75% of these patients. In 50% of the healthy women proinsulin and insulin secretion decreased. Obesity in pregnantpatients was associated with significant elevation of proinsulin, IRI, and C-peptyide levels and GDM with Lys/Lys genotype of polymorphous markerGlu23k of KCNJ11 gene, pro and ala allele of polymorphous marker A219T of ADIPOR2 gene. These associations suggest specific genetic featuresof GDM related to impaired insulin secretion and sensitivity.
Conclusion.
Studies of common genetic nature of GDM and DM2 permit to identify risk groups at the preclinical stage, plan prevention and treatmentof these disorders.
About the Authors
Tatiana Vasil'evna Sebko
Russian State Medical University, Moscow
Yulia Eduardovna Dobrokhotova
Russian State Medical University, Moscow
Tatiana Alekseevna Ivanova
Russian State Medical University, Moscow
Valery Vyacheslavovich Nosikov
State Research Institute of Genetics, Moscow
Tatiana Alekseevna Osipova
EFIS,Ltd, Moscow
Victor Andreevich Potapov
State Research Institute of Genetics, Moscow
References
1. American Diabetes Association: Gestational diabetes mellitus // Diabetes Care. - 1996. - 19 (Supp 1). - Р. 829.
2. Conway D.L., Langer O. «Elective delivery of infants with macrosomia in diabetes woman, reduced shoulder distocia versis increased delivery» //Am. J. Obstet. - 1998. - 78. - Р. 922-925.
3. Hod J. et all. Anterpartum management protocol timming and mode of delivery of gestational disordes // Diabetes Care. - 1998. - 21, Supp. 2. - Р. 8113- 9117.
4. Naylor C.D. ed all. «Cesarean delivery in relation to both weigt and gestational glycose tolerance: pathophisiology or practice style» // Jama. - 1996. - 275. - Р.1105-70.
5. Owen J. Gestational diabetes survey //Am. J. Obstet et Gynecol. - 1995. - 172. - Р. 615-620.
6. Moss R.G. et all. The recurrence of gestational diabetes: could dietary defferenses in Fat intake be an explanation // Diabetes Care. - 1997. - 20. - Р. 1647-50.
7. Silverman B.H. et all «Long term effects of the infraunterine environment» // Diabetes Care. - 1998. - 28: Supple 2. - Р. 13141- 13149.
8. Gestational diabetes mellitus // Diabetes Care. - 2000. - v22, supp. 1.
Review
For citations:
Sebko T.V., Dobrokhotova Yu.E., Ivanova T.A., Nosikov V.V., Osipova T.A., Potapov V.A. Genetic markers of insulin resistance in gestational diabetes. Diabetes mellitus. 2009;12(4):38-41. (In Russ.) https://doi.org/10.14341/2072-0351-5702
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