Abnormal Na-K-ATPase activity in erythrocytes from patients with diabetes mellitus
https://doi.org/10.14341/2072-0351-5399
Abstract
Aim.
To measure Na-K-ATPase activity in erythrocytes from patients with diabetes mellitus (DM), elucidate its relationship with clinical manifestationsof the disease and biochemical blood characteristics.
Materials and methods.
A total of 76 DM patients were enrolled in this study (39 with DM1 and 35 with DM2). Control group consisted of 11 subjectswithout DM. They were examined for HbA1c, nitrite and nitrate levels in blood and membranes, sialidase activity in erythrocytes and Na-KATPaseactivity measured from variations of inorganic phosphorus content in the presence of ATP.
Results.
Na-K-ATPase activity in MD patients was significantly lower than in controls (p<0.05). It was unrelated to the patients age and sex butdropped insignificantly with MD duration. Na-K-ATPase activity depended on blood HbA1c and membrane phosphorus levels, enhanced sialidaseactivity in erythrocytes, nitrite and nitrate concentrations in plasma.
Conclusion.
Na-K-ATPase activity reflects the degree of metabolic disturbances and endothelial dysfunction in patients with DM.
To measure Na-K-ATPase activity in erythrocytes from patients with diabetes mellitus (DM), elucidate its relationship with clinical manifestationsof the disease and biochemical blood characteristics.
Materials and methods.
A total of 76 DM patients were enrolled in this study (39 with DM1 and 35 with DM2). Control group consisted of 11 subjectswithout DM. They were examined for HbA1c, nitrite and nitrate levels in blood and membranes, sialidase activity in erythrocytes and Na-KATPaseactivity measured from variations of inorganic phosphorus content in the presence of ATP.
Results.
Na-K-ATPase activity in MD patients was significantly lower than in controls (p<0.05). It was unrelated to the patients age and sex butdropped insignificantly with MD duration. Na-K-ATPase activity depended on blood HbA1c and membrane phosphorus levels, enhanced sialidaseactivity in erythrocytes, nitrite and nitrate concentrations in plasma.
Conclusion.
Na-K-ATPase activity reflects the degree of metabolic disturbances and endothelial dysfunction in patients with DM.
About the Authors
Z M Shamansurova
НИИ Эндокринологии МЗ РУз, Ташкент
F A Mukhamedova
НИИ Эндокринологии МЗ РУз, Ташкент
A G Tsoi
НИИ Эндокринологии МЗ РУз, Ташкент
A B Tashmanova
НИИ Эндокринологии МЗ РУз, Ташкент
A V Alieva
НИИ Эндокринологии МЗ РУз, Ташкент
N A Akhrarova
НИИ Эндокринологии МЗ РУз, Ташкент
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Review
For citations:
Shamansurova Z.M., Mukhamedova F.A., Tsoi A.G., Tashmanova A.B., Alieva A.V., Akhrarova N.A. Abnormal Na-K-ATPase activity in erythrocytes from patients with diabetes mellitus. Diabetes mellitus. 2009;12(2):55-57. (In Russ.) https://doi.org/10.14341/2072-0351-5399
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