Urinary excretion of type IV collagen as an early marker of renal fibrosis in patients with diabetes mellitus
https://doi.org/10.14341/2072-0351-5813
Abstract
Aim.
To determine the diagnostic value of urinary excretion of type IV collagen in patients with type 1 diabetes with different stages of nephropathy.Methods.
Urinary type IV collagen was determined in 60 patients with type 1 diabetes (23 with normal albuminuria, 28 with microalbuminuriaand 9 with macroalbuminuria) by an enzyme immunoassay. 10 healthy individuals were acted as the control group. Renal biopsy was performedin 22 patients. Deposits of type IV collagen were revealed by 11 individuals by immunohistochemistry.
Results.
The urinary excretion of type IV collagen increased with severety of diabetic nephropathy, correlating with the urinary albumin/creatinineratio, serum creatinine and parameters of daytime and nighttime systolic and diastolic blood pressure. Patients with excessive accumulation of typeIV collagen in the glomeruli had significantly higher level of type IV collagen in the urine.
Conclusion.
The determination of urinary type IV collagen can be used for early detection of renal fibrosis in patients with type 1 diabetes.
To determine the diagnostic value of urinary excretion of type IV collagen in patients with type 1 diabetes with different stages of nephropathy.Methods.
Urinary type IV collagen was determined in 60 patients with type 1 diabetes (23 with normal albuminuria, 28 with microalbuminuriaand 9 with macroalbuminuria) by an enzyme immunoassay. 10 healthy individuals were acted as the control group. Renal biopsy was performedin 22 patients. Deposits of type IV collagen were revealed by 11 individuals by immunohistochemistry.
Results.
The urinary excretion of type IV collagen increased with severety of diabetic nephropathy, correlating with the urinary albumin/creatinineratio, serum creatinine and parameters of daytime and nighttime systolic and diastolic blood pressure. Patients with excessive accumulation of typeIV collagen in the glomeruli had significantly higher level of type IV collagen in the urine.
Conclusion.
The determination of urinary type IV collagen can be used for early detection of renal fibrosis in patients with type 1 diabetes.
About the Authors
Irina Arkad'evna Bondar'
Novosibirsk State Medical University, Novosibirsk
Vadim Valer'evich Klimontov
Novosibirsk State Medical University, Novosibirsk
Ekaterina Mikhailovna Parfent'eva
Novosibirsk State Medical University, Novosibirsk
Vyacheslav Vital'evich Romanov
Ltd. "INVITRO", Novosibirsk
Alexander Petrovich Nadeev
Novosibirsk State Medical University, Novosibirsk
References
1. Бондарь И.А., Климонтов В.В. Функциональная морфология почек при сахарном диабете. // Сахарный диабет и хроническая болезнь почек / Под ред. М.В. Шестаковой, И.И. Дедова. - М.: Медицинское информационное агентство, 2009. - С. 149-176.
2. Бондарь И.А., Климонтов В.В. Ранние маркеры диабетической нефропатии // Клиническая нефрология. - 2010. - № 2. - С. 60-65.
3. Kotajima N., Kimura T., Kanda T., Obata K., Kuwabara A., Fukumura Y., Kobayashi I. Type IV collagen as an early marker for diabetic nephropathy in non-insulin-dependent diabetes mellitus // J. Diabetes Complications. - 2000. - Vol. 14. - P. 13-17.
4. Okonogi H., Nishimura M., Utsunomiya Y., Hamaguchi K., Tsuchida H., Miura Y., Suzuki S., Kawamura T., Hosoya T., Yamada K. Urinary type IV collagen excretion reflects renal morphological alterations and type IV collagen expression in patients with type 2 diabetes mellitus // Clin. Nephrol. - 2001. - Vol. 55, № 5. - P. 357-364.
5. Araki S., Haneda M., Koya D., Isshiki K., Kume S., Sugimoto T., Kawai H., Nishio Y., Kashiwagi A., Uzu T., Maegawa H. Association between urinary type IV collagen level and deterioration of renal function in type 2 diabetic patients without overt proteinuria // Diabetes Care. - 2010. - Vol. 33, № 8. - P. 1805-1810.
6. Iijima T., Suzuki S., Sekizuka K., Hishiki T., Yagame M., Jinde K., Saotome N., Suzuki D., Sakai H., Tomino Y. Follow-up study on urinary type IV collagen in patients with early stage diabetic nephropathy // J. Clin. Lab. Anal. - 1998. - Vol. 12, № 6. - P. 378-382.
7. Бондарь И.А., Климонтов В.В., Королева Е.А., Желтова Л.И. Суточная динамика артериального давления у больных сахарным диабетом типа 1 с нефропатией // Пробл. эндокринол. - 2003. - № 5. - С. 5-10.
8. Furumatsu Y., Nagasawa Y., Shoji T., Yamamoto R., Iio K., Matsui I., Takabatake Y., Kaimori J. Y., Iwatani H., Kaneko T., Tsubakihara Y., Imai E., Isaka Y., Rakugi H. Urinary type IV collagen in nondiabetic kidney disease // Nephron. Clin. Pract. - 2011. - Vol. 117, № 2. - P. 160-166.
9. Дедов И. И., Шестакова М. В. Сахарный диабет и артериальная ги- пертензия. - М.: Медицинское информационное агентство, 2006. - С. 99-110.
10. Cohen M. P., Lautenslager G. T., Shearman C. W. Increased urinary type IV collagen marks the development of glomerular pathology in diabetic db/db mice // Metabolism. - 2001. - Vol. 50, № 12. - P. 1435-1440.
11. Cawood T.J., Bashir M., Brady J., Murray B., Murray P. T., O'Shea D. Urinary collagen IV and GST: potential biomarkers for detecting localized kidney injury in diabetes-a pilot study // Am. J. Nephrol. - 2010. - Vol. 32, № 3. - P. 219-225.
Review
For citations:
Bondar' I.A., Klimontov V.V., Parfent'eva E.M., Romanov V.V., Nadeev A.P. Urinary excretion of type IV collagen as an early marker of renal fibrosis in patients with diabetes mellitus. Diabetes mellitus. 2011;14(4):29-31. (In Russ.) https://doi.org/10.14341/2072-0351-5813
Views:
1826
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0).
Email this article 
