Activity of the adrenocortical system in rats with experimental diabetes
https://doi.org/10.14341/2072-0351-5632
Abstract
Aim.
To study the functional state of the adrenocortical system in experimental animals depending on severity of alloxan diabetes.
Materials and methods.
Diabetes in rats was induced by administering alloxan tetrahydrate at a dose of 17 mg/100 g b.w. Corticosteroids in plasma,adrenals, and 24-hr urine were measured by RIA, immunoenzyme assay, and HPLC. Hepatic aminotransferase activities were determined.
Results.
Durng the first week after induction of diabetes, the animals suffered metabolic disturbances and hypoinsulinemia the severity of which didnot significantly change up to day 30 Activation of adrenal glucocorticoid function (a rise in plasma corticosteron, urine and adrenal corticosteronand progesteron) occurred starting from days 8-9. Enhanced activity of hepatic aminotransferases confirmed physiological significance of elevatedblood corticosteron level.
Conclusion.
Physiological effects of glucocorticoids in the liver decreased by day 30 of experimental diabetes despite persisting disturbances in carbohydratemetabolism, probably due to reduced synthesis of corticosteron in adrenals and its concentration in blood.
To study the functional state of the adrenocortical system in experimental animals depending on severity of alloxan diabetes.
Materials and methods.
Diabetes in rats was induced by administering alloxan tetrahydrate at a dose of 17 mg/100 g b.w. Corticosteroids in plasma,adrenals, and 24-hr urine were measured by RIA, immunoenzyme assay, and HPLC. Hepatic aminotransferase activities were determined.
Results.
Durng the first week after induction of diabetes, the animals suffered metabolic disturbances and hypoinsulinemia the severity of which didnot significantly change up to day 30 Activation of adrenal glucocorticoid function (a rise in plasma corticosteron, urine and adrenal corticosteronand progesteron) occurred starting from days 8-9. Enhanced activity of hepatic aminotransferases confirmed physiological significance of elevatedblood corticosteron level.
Conclusion.
Physiological effects of glucocorticoids in the liver decreased by day 30 of experimental diabetes despite persisting disturbances in carbohydratemetabolism, probably due to reduced synthesis of corticosteron in adrenals and its concentration in blood.
About the Authors
Olga Pavlovna Cherkasova
Research Centre of Clinical and Experimental Medicine, Novosibirsk
Natalya Vladimirovna Kuznetsova
Research Centre of Clinical and Experimental Medicine, Novosibirsk
Natalya Alexandrovna Pal'chikova
Research Centre of Clinical and Experimental Medicine, Novosibirsk
Vera Georgievna Selyatitskaya
Research Centre of Clinical and Experimental Medicine, Novosibirsk
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Review
For citations:
Cherkasova O.P., Kuznetsova N.V., Pal'chikova N.A., Selyatitskaya V.G. Activity of the adrenocortical system in rats with experimental diabetes. Diabetes mellitus. 2011;14(2):37-40. (In Russ.) https://doi.org/10.14341/2072-0351-5632
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