diaendoСахарный диабетDiabetes mellitus2072-03512072-0378Endocrinology research centre10.14341/DM8039diaendo-8039Research ArticleЭкспериментальная диабетологияExperimental DiabetologyПигмент эхинохром ослабляет проявления диабетической нефропатии на экспериментальных моделях сахарного диабета 1 и 2 типовEchinochrome pigment attenuates diabetic nephropathy in the models of type 1 and type 2 diabetesSolimanAmel M.SolimanAmel Mahmoud

Professor of Physiology, Zoology Department

soliman.amel5@gmail.comMohamedAyman S.MohamedAyman Saberayman81125@hotmail.comMarieMohamed-Assem S.MarieMohamed-Assem Saidmassemmarie@yahoo.comКаирский университетЕгипетCairo UniversityEgypt201602122016196464470Copyright © Soliman A.M., Mohamed A.S., Marie M.S., 20162016Soliman A.M., Mohamed A.S., Marie M.S.Soliman A.M., Mohamed A.S., Marie M.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.dia-endojournals.ru/jour/article/view/8039Актуальность

Актуальность. Одним из главных осложнений сахарного диабета обоих типов является диабетическая нефропатия, которая выступает основной причиной почечной недостаточности. Пигмент эхинохром, содержащийся в раковинах и иголках морских ежей, обладает высокой антиоксидантной активностью.

Цель

Цель. Изучить способность эхинохрома замедлять прогрессирование диабетической нефропатии.

Материалы и методы

Материалы и методы. Тридцать шесть крыс-альбиносов линии Wistar мужского пола были разделены на 2 группы: группа сахарного диабета 1 типа (СД1) и группа сахарного диабета 2 типа (СД2). Обе группы были, в свою очередь, разделены на подгруппы: группу контроля, группу диабета и группу эхинохрома. СД1 у мышей вызывали однократным введением стрептозотоцина (60 мг/кг интраперитонеально), а СД2 — повышенным содержанием жиров в пище в течение 4 недель до введения стрептозотоцина (30 мг/кг интраперитонеально). Экспериментальным группам вводили эхинохром (1 мг/кг в 10% растворе диметилсульфоксида) ежедневно в течение 4 недель.

Результаты

Результаты. В группах эхинохрома было отмечено снижение концентрации глюкозы, малондиальдегида, мочевины, мочевой кислоты и креатинина. Было также отмечено повышение уровней глутатион-S-трансферазы, супероксиддисмутазы, каталазы, восстановленного глутатиона, оксида азота и клиренса креатинина. Гистологические исследования показали отчетливое восстановление микроархитектоники почечной ткани.

Background. The main complication of diabetes mellitus is diabetic nephropathy in both types, which is a main reason for renal failure. Echinochrome substance present in sea urchin shells and spines and possesses high antioxidant activity.Aim. is to evaluate the ability of Ech to suppress the progression of diabetic complication in kidney.Materials and methods. Thirty-six male Wistar albino rats were divided into two main groups, type 1 diabetes mellitus and type 2 diabetes mellitus. Both groups divided into control, diabetic and echinochrome subgroups. Type 1 diabetes was induced by single dose of streptozotocin (60 mg/kg, i.p), while type 2 was induced by high fat diet for 4 weeks before the injection with streptozotocin (30 mg/kg, i.p). The treated groups were administrated by echinochrome (1mg/kg body weight in 10% DMSO) daily for 4 weeks.Results. Echinochrome groups showed reduction in the concentrations of glucose, malondialdehyde, urea, uric acid and creatinine. While it caused general increase in glutathione-S-transferase, superoxide dismutase, catalase, glutathione reduced, nitric oxide and creatinine clearance. The histopathological investigation showed clear improvement in the kidney architecture.Conclusion. Administration of echinochrome improves renal function and ameliorates renal histopathological changes possibly by improvement of glucose metabolism and inhibition of lipid peroxidation process.

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The authors declare that there are no conflicts of interest present.