diaendoСахарный диабетDiabetes mellitus2072-03512072-0378Endocrinology research centre10.14341/DM2014377-82diaendo-6496Research ArticleДетская диабетологияPediatric diabetologyФункциональное состояние нейтрофильных гранулоцитов и маркеры апоптоза при сахарном диабете 1 типа у детейNeutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetesБарычеваЛюдмила ЮрьевнаBarychevaLyudmila Yur'evna

доктор медицинских наук, профессор кафедры детских инфекционных болезней

MD, PhD, Professor of the Chair of Pediatric Infectious Diseases

for_ludmila@inbox.ruЭрдни-ГоряеваНаталья ЭдуардовнаErdni-GoryaevaNatalya Eduardovna

MD, Endocrinologist of the Stavropol Regional Clinical Diagnostic Center; Competitor of the Chair of Pediatric Infectious Diseases

for_ludmila@inbox.ruАлександровичГалина АлексеевнаAlexandrovichGalina Alekseevna

кандидат медицинских наук, доцент, заслуженный врач России, заведующий кафедрой эндокринологии, детской эндокринологии и диабетологии

MD, PhD, Associate Professor, Honored Doctor of Russia, Head of the Chair of Endocrinology, Pediatric Endocrinology, and Diabetology

for_ludmila@inbox.ruГБОУ ВПО Ставропольский государственный медицинский университет, СтавропольРоссияStavropol State Medical University, StavropolRussian Federation2014140620141737782Copyright © Барычева Л.Ю., Эрдни-Горяева Н.Э., Александрович Г.А., 20142014Барычева Л.Ю., Эрдни-Горяева Н.Э., Александрович Г.А.Barycheva L.Y., Erdni-Goryaeva N.E., Alexandrovich G.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.dia-endojournals.ru/jour/article/view/6496

В последние годы доказано, что полиморфноядерные лейкоциты играют важную роль в развитии сахарного диабета (СД). Дисфункция нейтрофилов способствует повреждению ткани поджелудочной железы, а также повышенной восприимчивости к инфекции при СД 1 типа (СД1). Цель. Изучение функциональной активности нейтрофильных гранулоцитов (НГ) при СД1 у детей. Материалы и методы. Обследовано 25 детей в возрасте от 7 до 15 лет. Для оценки программируемой клеточной гибели выявляли количество НГ, экспрессирующих маркеры апоптоза (CD95, CD95L, Bcl2). Функциональную активность НГ определяли по показателям фагоцитоза, уровню миелопероксидазы, лизосомальных катионных белков, активных радикалов кислорода. Результаты. Установлено снижение бактерицидной активности НГ с дефицитом поглощения, секреции активных радикалов кислорода, функционального резерва. Показано увеличение готовности к апоптозу, что сопровождалось повышением экспрессии CD95, снижением ? Bсl2. Выявлено увеличение цитотоксического потенциала нейтрофилов в виде повышения уровня миелопероксидазы и лизосомальных катионных белков. Заключение. Увеличение апоптотического потенциала НГ на фоне функционально-метаболических изменений может являться отражением их активного вовлечения в иммунопатогенез заболевания.

In recent years, polymorphonuclear leukocytes have been proven to play an important role in the development of diabetes mellitus (DM). Neutrophil dysfunction contributes to pancreatic tissue damage as well as an increased susceptibility to infection in type 1 DM (T1DM) patients. Objective. The objective of this study was to investigate the functional activity of neutrophil granulocytes (NGs) in children with T1DM. Materials and Methods. This study involved 25 children aged 7?15 years. To evaluate programmed cell death, the number of NGs expressing apoptosis markers (CD95, CD95L and BCL2) was determined. The functional activity of NGs was determined in terms of phagocytosis and the levels of myeloperoxidase, lysosomal cationic proteins and active oxygen radicals. Results. A reduction in the bactericidal activity of NGs with deficiencies in phagocytosis, secretion of active oxygen radicals and functional reserve was found. An increase in the apoptotic potential of NGs was demonstrated, which was accompanied by an increase in CD95 expression and a decrease in BCL2 expression. An increase in the cytotoxic potential of neutrophils in the form of enhanced levels of myeloperoxidase and lysosomal cationic proteins was revealed. Conclusions. Therefore, an increase in the apoptotic potential of NGs associated with functional and metabolic changes may reflect the active involvement of NGs in the immunopathogenesis of T1DM.

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The authors declare that there are no conflicts of interest present.