diaendoСахарный диабетDiabetes mellitus2072-03512072-0378Endocrinology research centre10.14341/2072-0351-5631diaendo-5631Research ArticleСтатьиArticlesВлияние высокой концентрации глюкозы на способность мезенхимальных стромальных клеток контролировать рост кровеносных сосудовThe influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growthАкопянЖанна АлексеевнаAkopyanZhanna Alekseevnasecretary@fbm.msu.ruШароновГеоргий ВладимировичSharonovGeorgy Vladimirovich-КочегураТатьяна НиколаевнаKocheguraTatiana Nikolaevna-ИльяшенкоНаталья ФедоровнаIl'yashenkoNatalya Fedorovna-БелянкоИгорь ЭдуардовичBelyankoIgor Eduardovich-ДимитроваВалентина ИвановнаDimitrovaValentina Ivanovna-ЗотиковАндрей ЕвгеньевичZotikovAndrey Evgen'evich-КалининаНаталья ИгоревнаKalininaNatalya Igorevna-ПарфёноваЕлена ВикторовнаParfenovaElena Viktorovna-ГУНУ Факультет фундаментальной медицины МГУ имени М.В. Ломоносова, МоскваDepartment of Fundamental Medicine, M.V.Lomonosov Moscow State University, Moscow201115062011142№2 (2011)3235Copyright © Акопян Ж.А., Шаронов Г.В., Кочегура Т.Н., Ильяшенко Н.Ф., Белянко И.Э., Димитрова В.И., Зотиков А.Е., Калинина Н.И., Парфёнова Е.В., 20112011Акопян Ж.А., Шаронов Г.В., Кочегура Т.Н., Ильяшенко Н.Ф., Белянко И.Э., Димитрова В.И., Зотиков А.Е., Калинина Н.И., Парфёнова Е.В.Akopyan Z.A., Sharonov G.V., Kochegura T.N., Il'yashenko N.F., Belyanko I.E., Dimitrova V.I., Zotikov A.E., Kalinina N.I., Parfenova E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.dia-endojournals.ru/jour/article/view/5631

Жировая ткань (ЖТ) является источником мезенхимальных стволовых клеток (МСК), которые могут быть использованы для стимуля-ции роста кровеносных сосудов в ишемизированных тканях. Однако различные метаболические расстройства, включая гипергликемию,могут отрицательно влиять на ?терапевтические? свойства этих клеток.Цель. Изучение влияния высокой концентрации глюкозы на функциональную активность МСК из ЖТ (МСК-ЖТ) человека. Материалы и методы. С помощью методов проточной цитометрии и ПЦР в реальном времени исследована функциональная активностькультивируемых МСК из ЖТ человека в условиях инкубации в среде с высокой концентрацией глюкозы. Результаты. Показано, что длительная (в течение 10-12 дней) инкубация в среде с высокой концентрацией глюкозы подавляла спо-собность МСК стимулировать ангиогенез in vitro. При этом в клетках, культивированных в присутствии 25мМ глюкозы, изменяласьэкспрессия генов активаторов и ингибиторов ангиогенеза. Однако пролиферация и апоптоз МСК при инкубации в данных условияхне изменялись. Заключение. Высокая концентрация глюкозы вызывает подавление ангиогенной активности МСК-ЖТ, что может являться причинойнедостаточного восстановления кровотока у пациентов с сахарным диабетом.

Adipose issue is a source of mesenchymal stem cells (MSC) that can be used to stimulate blood vessel growth in ischemic tissues. Various metabolicdisorders including hypeglycemia may have negative effect on therapeutic properties of these cells. Aim. To study the influence of high glucose concentration on functional activity in human adipose tissue. Materials and methods. Flow cytometry and real time PCR were used to study functional activity of cultured MSC from human adipose issue at highglucose concentration. Results. Prolonged (10-12 days) incubation at a high glucose concentration (25 mM) suppressed the ability of MSC to stimulate angiogenesis. Also,glucose modified expression of genes activating and inhibiting angiogenesis but had no effect on MSC proliferation and apoptosis. Conclusion. High glucose concentration suppresses angiogenic activity of MSC in adipose tissue; it may account for incomplete restoration of bloodflow in diabetic patients.

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The authors declare that there are no conflicts of interest present.