diaendoСахарный диабетDiabetes mellitus2072-03512072-0378Endocrinology research centre10.14341/DM13279diaendo-13279Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL STUDIESРоль тиосульфат-сульфуртрансферазы в окислительном стрессе при сахарном диабете 2 типаThe Role of Thiosulfate Sulfurtransferase in Oxidative Stress for Type 2 Diabetes Mellitushttps://orcid.org/0009-0003-0918-9743МавадждеМ. М.MawajdehM. M.

Марва М. Маваджде

Мосул

Marwa M. Mawajdeh - Master’s student in Biochemistry.

Mosul

marwa.23scp80@student.uomosul.edu.iqhttps://orcid.org/0000-0002-3479-8001АллушТ. А.AllwshT. A.

Тикра А. Аллуш

Мосул

Thikra A. Allwsh - Professor, PhD in Biochemistry; ResearcherID: https://www.researchgate.net/profile/Thikra-Allwsh; Scopus Author ID: 57226087609.

Iraq, Mosul, Al-Zahraa Street, 41003

thekraaliallwsh@uomosul.edu.iqУниверситет МосулаИракUniversity of MosulIraq202509102025284359366Copyright © Маваджде М.М., Аллуш Т.А., 20252025Маваджде М.М., Аллуш Т.А.Mawajdeh M.M., Allwsh T.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.dia-endojournals.ru/jour/article/view/13279ОБОСНОВАНИЕ

ОБОСНОВАНИЕ. Сахарный диабет 2 типа (СД2) ассоциирован с окислительным стрессом, который приводит к инсулинорезистентности. Тиосульфат-сульфуртрансфераза (ТСТ) — это митохондриальный фермент, участвующий в реакциях с цианидом, эндогенным сероводородом (H₂S) и активными формами кислорода.

ЦЕЛЬ

ЦЕЛЬ. Целью данного исследования было изучение взаимосвязи между ферментом ТСТ и маркерами как окислительного, так и антиоксидантного стресса у пациентов с СД2. Предполагается, что ТСТ связана с окислительным стрессом, который играет ключевую роль в определении тяжести и прогрессирования заболевания.

МАТЕРИАЛЫ И МЕТОДЫ

МАТЕРИАЛЫ И МЕТОДЫ. В исследование «случай-контроль» включены 150 пациентов с СД2, принимавших метформин (Глюкофаж) по 500 мг дважды в день, а также 150 здоровых лиц в возрасте от 33 до 65 лет. Активность ТСТ оценивалась на основе переноса серы и образования тиоцианата. Также измерялись уровни малонового диальдегида (МДА), пероксинитрита, пероксидазы, арилэстеразы, витамина C, витамина E, тиоредоксина (Trx) и глутатиона (GSH). Помимо клинических маркеров, все измерения проводились в двух повторах, был выполнен статистический анализ, а данные представлены в виде медианы и межквартильного размаха.

РЕЗУЛЬТАТЫ

РЕЗУЛЬТАТЫ. Активность ТСТ была значительно ниже (на 55%) у пациентов с СД2 по сравнению с контрольной группой (8,5 (3,8) против 19 (2) Ед/мл соответственно). Была обнаружена обратная зависимость между активностью фермента и возрастом, в то время как у курящих активность фермента была выше. Уровни антиоксидантных соединений, таких как витамин С, витамин Е, GSH, Trx, и активность арилэстеразы были значительно ниже, в то время как маркеры окислительного стресса, включая активность пероксидазы, МДА и пероксинитрит, были значительно выше. Активность ТСТ показала отрицательную корреляцию с МДА и пероксинитритом и положительную корреляцию с Trx и GSH.

ЗАКЛЮЧЕНИЕ

ЗАКЛЮЧЕНИЕ. Активность ТСТ снижена у пациентов с СД2 и связана с окислительным стрессом. Это позволяет предположить, что ТСТ может играть защитную роль против окислительного стресса, что делает ее потенциальным индикатором метаболической регуляции и возможной терапевтической мишенью.

Полный текст статьи доступен в электронной версии журнала на сайте www.dia-endojournals.ru

BACKGROUND

BACKGROUND: Type 2 diabetes mellitus (T2DM) is associated with oxidative stress, leading to insulin resistance. Thiosulfate sulfurtransferase (TST) is mitochondrial enzyme involved in the reaction with cyanide, endogenous hydrogen sulfide (H₂S), and reactive oxygen species.

AIM

AIM: This study aimed to investigate the relationship between TST enzyme and both oxidative and anti-oxidative stress markers in T2DM patients. TST is believed to be related to oxidative stress, which plays a crucial role in determining the severity and progression of the disease.

MATERIALS AND METHODS

MATERIALS AND METHODS: A case-control study included (150) T2DM patients who were taking the drug Metformin (Glucophage) 500 mg twice daily as well as (150) healthy subjects aged between 33 to 65 years. TST activity was estimated based on the sulfur transfer and thiocyanate formation. Malonaldehyde (MDA), peroxynitrite, peroxidase, aryl esterase, vitamin C, vitamin E, thioredoxin (Trx) and glutathione (GSH) were also measured. In addition to clinical markers, all measurements were made in two replicates, statistical analyses were conducted, and data were presented as a median and interquartile range.

RESULTS

RESULTS: TST activity was significantly lower (by 55%) in T2DM patients compared to the controls (8.5 (3.8) vs. 19 (2) U/ml, respectively). There was an inverse relationship between enzyme activity and age, whereas enzyme activity increased with smoking. Antioxidant compounds such as vitamin C, vitamin E, GSH, Trx, and arylesterase activity were significantly lower, while oxidant markers including peroxidase activity, MDA, and peroxynitrite, were significantly higher. TST activity showed a negative correlation with MDA and peroxynitrite, and a positive correlation with Trx and GSH.

CONCLUSION

CONCLUSION: TST activity is reduced in T2DM patients and is associated with oxidative stress. This suggest that TST may play a protective role against oxidative stress, making it a potential indicator of metabolic regulation and a possible therapeutic target.

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The authors declare that there are no conflicts of interest present.